Phage size does Matter…

It represents the maximum potential capacity of the insertion length (bp).  Small phage particle cannot afford the over-size DNA cloning.  

 

publishcation

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2). Ren, Z.J., et al. (1997)   Cloning of linear DNAs in vivo by overexposed T4 DNA ligase: construction of a T4 phage hoc gene display vector. Gene, 195: 303-311.     

3). Ren, Z.J., and Black, L.W (1998): Phage T4 SOC and HOC display of biologically active, full-length proteins on the viral capsid. Gene, 215 :439.

4). Wu,J.M.,et. al..(2004): Bacteriophage T4 capsid surface Soc and Hoc sites bipartite display: CSFV immunogenicity comparison with plasmid expressed antigen. (submitted to Infection and Immunity)

5). Ren,Z.J, et al.: Development of  a novel FMDV oral vaccine: T4 phage-engineering with full-length capsid gene P1 and proteinase gene 3C both expressed on viron surfice. (Vaccine, in preparation )

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